刺五加注射液对人肺腺癌A549细胞增殖的抑制作用
Inhibitory effect of Acanthopanax senticosus injection on growth of human lung adenocarcinoma A549 cells
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摘要: 目的 探讨刺五加注射液对人肺腺癌A549细胞的体外增殖的抑制作用。方法 采用MTT法测定了不同浓度的刺五加注射液对人肺腺癌A549细胞的体外抑制作用,倒置显微镜观察细胞形态变化。结果 刺五加注射液浓度越高对A549细胞抑制作用越好,作用时间越长抑制效果越强,量效关系和时效关系良好;形态学观察到细胞凋亡。结论 刺五加注射液对人肺腺癌A549细胞的增殖有较强的抑制作用。Abstract: ObjectiveTo explore the inhibitory effect of Acanthopanax senticosus injection on the growth of human lung adenocarcinoma A549 cells.MethodsMTT assay was used to determine the growth of lung adenocarcinoma A549 cells and inhibitory rates of Acanthopanax senticosus injection at different concentrations,and to observe the change in cell shape under an inverted microscope.ResultsAcanthopanax senticosus injection at a higher concentration had a stronger inhibitory effect on the growth of human lung adenocarcinoma A549 cells,longer action time,and a better dose-effect and time-effect relation.Apoptosis of human lung adenocarcinoma A549 cells was found during morphological observations.ConclusionAcanthopanax senticosus injection has a stronger inhibitory effect on the growth of human lung adenocarcinoma A549 cells.
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