Abstract: Objective: To explore the effects of LRP16 gene on glucose-stimulated insulin secretion（GSIS）and the possible mechanism in MIN6 cell line. Methods: Stable overexpression of LRP16 cell models was established by SuperFect strategy.The effects of LRP16 overexpression on MIN6 cell proliferation were examined by MTT method.Different concentrations of glucose were used to evaluate the effects of GSIS in the transfected cells.The expression levels of GLUT-2 in MIN6 cells were analysed by western blot. Results: Overexpression of LRP16 did not improve cell proliferation compared with control group（P＞0.05）,but it increased GSIS by 2.26,2.19 and 2.16 folds under 0mmol/L,3mmol/L and 30mmol/L glucose concentrations respectively,and western blot analyses showed that the levels of GLUT-2 in LRP16 overexpression group increased significantly compared with control group（P＜0.05）. Conclusion: Overexpression of LRP16 can not improve cell proliferation,but it can increase GSIS by up-regulating GLUT-2 in MIN6 cell line（P＜0.05）.This may indicate that the effects of LRP16 on GSIS do not rely on cell proliferation but on increased uptake of glucose.