Abstract: Objective: To investigate the expression of Phoenix and HepG2 cells which were spin-infected by retrovirus with human insulin gene.Methods: To transfect the pM54,p54 and pCMVβGal plasmids into pT67 and Phoenix package cell lines by FuGENE Method;3T3 cell was control;Supernatants were collected after transfection 48 or 72 hours with DNA;Spin-infected Phoenix and HepG2 cells by retrovirus;supernatants were collected at 72 hours post-infection;Check the expression of INS by ELISA.Results: ELISA method detecting proved that the human insulin protein was expressed in the supernatants of spin-infected HepG2 and Phoenix cells.All the controls were negative.Conclusion: This study provide a vital data for the further research of the insulin gene elements,which was modified to intervene the stem cell insulin production to treat diabetes related subject.