Abstract: Objective: To study the effects of Fructose-1,6-diphosphate（FDP） on Endotoxin（ET）-induced acute lung injury（ALI） and the activities of phospholipase A 2（PLA₂） in rabbits. Methods: Japanese flap-eared white rabbits were randomly assigned to three groups: control group （group A）, ET-treated group （group B） and combination group（treated by ET and FDP, group C）.Acute lung injury was induced by the injection of ET at a time. During the experiment, respiratory rates, heart rates, blood pressure, arterial blood gases and the plasma PLA₂ activities were measured at 0h, 0.5h, 2h, 4h and 6h respectively. Then, after rabbits being killed at 6h, the pulmonary PLA₂ activities were measured, and the pathologic changes were examined with light microscope. Results: Compared with group A, rabbits of group B showed the typical characters of ALI, including respiratory rates and heart rates increasing, as well as blood pressure and arterial blood oxygen pressure decreasing. Significant increase of the PLA₂ activities was also seen both in blood serum and lung tissue in group B. Conditions in group C were better than those in group B. Conclusion: FDP can inhibit the PLA₂ activities, improves its pathologic physiological procedure, and possesses protective effects on early ALI in rabbits induced with ET.