抗Lewisα单克隆抗体CS3识别肿瘤细胞表面抗原位点的流式细胞术(Flowcy tometry)添加试验分析

Flow Cytometry-additivity test analysis of anti-Lewisα monoclonal antibodies CS3recognizing antigenic sites on the tumor cell

  • Abstract: In this paper,we described a Flow Cytometry-additivity test to analyze the monoclonal antib-odies to recognize different antigenic determinants on the tumor cells.The target cells are mixed.with two antibodies either separately or simulteneously,and are stained with FITC-Ig conjugate.The amount of bound antibody is quntitatively measured by the use of Flow Cytometry.AI (ad-ivity index) is determined using a computer program developed for curve fitting of Flow Cyto etry.Using this test,it has been shown that the monoclonal antibodies Lewis a,CS3,can be reco-gnized at the same sites on two tumor cell line used.

     

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