中国维吾尔族和苗族人群线粒体DNA 9-bp缺失频率

Frequence of 9-bp delection in the human mitochondrial DNA among the Weiwuer nationality and Miao nationality in China

摘要: 目的: 了解了中国新疆维吾尔族和湖北恩施地区苗族线粒体DNACOII tRNA^Lys基因间小非编码区Ⅴ串联重复序列9-bp缺失频率。方法: 应用PCR及DNA序列分析技术。结果: 研究发现,80例新疆维族人中有2例(2. 5%)有mtDNA 9-bp缺失;4 7例湖北恩施地区苗族人中有5例(10. 6%)有mtDNA 9-bp缺失。结论: 研究结果表明中国少数民族的维族、苗族人群中存在mtDNA 9-bp缺失多态性,而其缺失频率差别较大。

Abstract: Objective: To search the intergenic COII/tRNA^Lys 9-bp deletion in the human mitochondrial DNA (mtDNA) among the Weiwuer nationality and Miao nationality in China. Methods: The DNA fragment in mtDNA intergenic COII/tRNA^Lys was amplified by PCR technique. Some samples were analysed by direct sequencing of PCR products. Results: We screened the present 80 Weiwuer nationality and 47 Miao nationality for the 9-bp deletion. The frequency of the 9-bp deletion was 2. 5% for Weiwuer nationality,10. 6% for Miao nationality. All this deletive cases were identified in this region present lack one of two adjacent copies of a 9-bp sequence normally. Conclusion: The data obtioned indicate that the intergenic COII/tRNA^Lys 9-bp deletion were discovered in Weiwuer nationality and Miao nationality with varying frequencies.