紫杉醇联合肿瘤坏死因子α对腺样囊性癌细胞的抑制作用

董青; 杨湲波; 王琳; 吴文慧; 白宇宏

紫杉醇联合肿瘤坏死因子α对腺样囊性癌细胞的抑制作用

Study of apoptosis of salivary adenoid cystic carcinoma induced by TNF-α and paclitaxel in vitro

摘要

摘要: 目的:探讨人肿瘤坏死因子α(TNF-α)联合紫杉醇对人腺样囊性癌细胞系SACC-83的抑制增殖作用及与细胞凋亡的关系,为化疗与生物治疗联合应用治疗腺样囊性癌提供实验依据。方法:将人腺样囊性癌细胞系SACC-83和对照组原代培养鼠肺成纤维细胞根据不同药物浓度分为9组,药物作用72 h。³H-TDR法检测细胞增殖抑制率;流式细胞术检测细胞凋亡率及细胞周期改变;透射电镜观察凋亡细胞形态变化。结果:联合用药组的细胞增殖抑制率显著高于单独用药组(P<0.05)。L929细胞各组的细胞增殖抑制率显著低于SACC-83细胞(P<0.05)。结论:TNF-α联合紫杉醇对SACC-83细胞增殖的抑制作用以及诱导凋亡具有协同作用。

Abstract: Objective: To explore the apoptosis mechanism of salivary adenoid cystic carcinoma cells（SACC-83） induced by recombinant human tumor necrosis factor α（rhTNF-α） and paclitaxel.Methods: The SACC-83 cells and primary culture of L929 cells were divided into nine groups of different medicine concentration and exposed for 72h.The inhibition ratio of cell proliferation was detected with ³H-TDR.The percentage of apoptotic cells and cell cycle distribution were detected by the flow cytometer.The cell morphological change of apoptosis was observed.Results: The inhibition ratio of cell proliferation in associated drugs group was significant higher than that of either single drug group（P＜0.05）.The inhibition ratio of cell proliferation of every group of L929 cell was significant lower than that of SACC-83 cell（P＜0.05）.Conclusion: TNF-α associated with paclitaxel is synergetic to inhibit SACC-83 cells and induce apoptosis.

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