Abstract: Objective To investigate the distribution of clinical isolates from blood,urine and other fluids and their drug resistance monitoring in 9 military hospitals of China in 2007. Methods A total of 1 397 non-repetitive clinical isolates were collected and sent to the Chinese PLA General Hospital for re-identification and susceptibility testing.A minimal inhibitory concentration（MIC）of antimicrobial agents and ESBL-producing bacteria were detected using the agar dilution method.WHONET5.4 software was used to analyze the data. Results Gram-positive cocci and Gram-negative bacilli accounted for 27.3%and 72.7%respectively of the 1 397 clinical isolates from blood,urine and other fluids.The detection rate of methicillin-resistant staphylococcus aureus（MRSA）was 41.3%.The susceptible rate of MRSA was lower than 13%to antimicrobial agents and 93.9%-100%to vancomycin,teicoplanin and minocycline,respectively.No isolate of Enterococci and Staphylococcus aureus was resistant to vancomycin.The susceptibility rate of meropenem and imipenem to ciprofloxacin was 100%,while that of Escherichia coli and Klebsiella spp to ciprofloxacin was only 12%.ESBL-producing strains accounted for 69.4%and 61.8%in Escherichia coli and Klebsiella spp,respectively.The multidrug resistance of P.aeruginosa（MDRPA）and Acinetobacter baumannii（MDRA）was 34.6%and 60.2%,respectively.The resistance of Escherichia coli isolated from urine to ciprofloxacin and levofloxacin was 89.6%and 90.6%,respectively. Conclusion The resistance levels in isolates of MRSA,ESBLs,MDRPA and MDRA to antimicrobial agents are high in military hospitals of China.The resistance of bacteria isolated from urine to fluorquinolone agents is increasing.