抗-HCV不同试剂检测结果分析

Detection of anti-HCV antibody with different methods

摘要: 目的探讨不同检测方法、试剂对丙型肝炎病毒(HCV)抗体检测结果的影响。方法以不同试剂采用酶联免疫吸附试验(ELISA)对献血者血浆标本进行抗-HCV检测,呈反应性标本采用HCV RNA RT-PCR荧光定量确证检测。结果 ELISA再次检测抗HCV抗体阳性率明显低于初次检测阳性率,差异有统计学意义(P<0.01),确证试验阳性率低于初次检测和再次检测的阳性率,差异有统计学意义(P<0.05)。结论 ELISA测定结果为HCV抗体阳性者应经HCV RNA RT-PCR荧光定量加以确认。

Abstract: Objective To investigate the impact of different methods and reagents on detection of anti-HCV antibody in blood donors. Methods Anti-HCV antibody in blood donors was detected by enzyme-linked immunosorbent assay（ELISA）using different reagents,and positive sera were examined by Western blotting confirmatory test. Results Western blotting confirmatory test showed a lower positive anti-HCV rate than ELISA（P＜0.05）. Conclusion Positive HCV antibody detected by ELISA should be confirmed by quantifying fluorescence in HCV RNA RT-PCR.