Abstract: Objective To explore the feasibility and clinical significance of mediastinal tumors resection by single utility port videoassisted thoracoscopic surgery. Methods From August 2006 to November 2009,32 cases underwent mediastinal tumor resection with single utility port video-assisted thoracoscopic surgery.Of all patients,thymoma was 15 cases,thymic cysts 6 cases,neurogenic tumor 4 cases,teratoid tumor 3 cases,bronchogenics cyst 3 cases,pericardium cysts 1 cases. Results The operation process were smooth in all patients and no conversion to thoracotomy was occurred.The mean operative time was（92.7±16.2）min and the mean intraoperative blood loss was（110.5±24.6）ml.The average chest tube drainage time was（2.1±1.3）days,and the mean hospital stay was（5.2±1.2）days.All patients recovered smoothly without severe complications.There were no post-operative deaths. Conclusions Mediastinal tumor resection by single utility port video-assisted thoracoscopic surgery is technically feasible and has the advantages of minimal invasive and rapid recovery.