激光扫描共聚焦显微镜研究甲状腺细胞间隙连接通讯

A method of gap junctional intercellular communication about FRTL-5 cell with laser scanning confocal microscope

摘要: 目的：建立甲状腺细胞细胞间隙连接通讯的研究方法。方法：运用激光扫描共聚焦显微成像仪，甲状腺细胞经培养后，用CFDA染色，用荧光光漂白后的恢复技术测定荧光恢复速度。比较不同剂量的白介素-1（IL-1），肿瘤坏死因子-1、肿瘤坏死因子-α），对甲状腺细胞细胞间隙连接通讯的影响。结果：白介素-1、肿瘤坏死因子-α对甲状腺细胞细胞间隙连接通讯有抑制作用。结论：本方法具有准确、快速、操作简便的优点，将成为新的研究手段。

Abstract: Objective:A Method of gap junctional intercellular communication about FRTL-5 cell with laser scanning confocal microscope was established.Methods:After RFTL-5 cell incubated with CFDA-AM, the fluorescence recovery after photobleaching（FRAP） techniques was used Results:With different dosages of interleukin-1 and tumor necrosis factor-a, FRTL-5 cell's gap junctional intercellular communication were restrained.Conclusion:The results indicate that it is a sensitive, quick and simple method. in gap junctional intercellular communication research.