Raf/MEK/ERK信号通路对CXCL16诱导的CIA淋巴细胞增殖的影响
Effect of Raf/MEK/ERK signal pathway on CXCL16-induced proliferation of lymphocytes in CIA mice
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摘要: 目的 探讨细胞外信号调节激酶(ERK)信号通路在CXCL16诱导的CIA小鼠淋巴细胞增殖中的调控作用。方法 CCK8法检测CXCL16对CIA小鼠淋巴细胞增殖的影响,ELISA法检测细胞上清中TNF-α及RANKL表达,WesternBlot检测p-Raf-1、p-ERK1/2蛋白的表达,同时观察MEK抑制剂对CXCL16诱导的上述变化的影响。结果 CXCL16对正常小鼠淋巴细胞增殖的刺激作用不明显,但CIA+CXCL16组淋巴细胞增殖能力明显高于CIA空白组及CIA+CXCL16+抑制剂组(P<0.05)。CIA+CXCL16组TNF-α水平明显高于其他各组(P<0.01)。RANKL水平在CIA+CXCL16组明显高于CIA空白组(P<0.01),但与CIA+CXCL16+抑制剂组相比无明显差别。p-Raf-1/β-actin及p-ERK1/2/β-actin在CIA+CXCL16组明显高于CIA空白组(P<0.05)。结论 CXCL16可能通过激活Raf/MEK/ERK信号通路,诱导CIA小鼠淋巴细胞增殖活化。Abstract: Objective To study the effect of Raf/MEK/ERK signal pathway on CXCL16-induced proliferation of lymphocytes in CIA mice. Methods Effect of CXCL16 on proliferation of lymphocytes in CIA mice was detected by CCK8 assay.Expressions of TNF-α and RANKL as well as p-Raf-1 and p-ERK1/2 protein in cell supernatant were detected by ELISA and Western blotting,respectively.Effect of MEK inhibitors on CXCL16-induced changes in expression of TNF-α and RANKL as well as p-Raf-1 and p-ERK1/2 protein in cell supernatant was observed. Results CXCL16 had no significant effect on proliferation of lymphocytes in normal mice.The proliferation level of lymphocytes was significantly higher in CIA+CXCL16 group than in CIA blank and CIA+CXCL16+inhibitor groups（P＜0.05）.The TNF-α level was significantly higher in CIA+CXCL16 group than in other groups（P＜0.01）.The RANKL level was significantly higher in CIA+CXCL16 group than in CIA blank group（P＜0.01） with no significant difference between CIA+CXCL16 and CIA+CXCL16+inhibitor groups.The expression level of p-Raf-1 and p-ERK1/2 was significantly higher in CIA+CXCL16 group than in CIA blank group（P＜0.05）. Conclusion CXCL16 can induce proliferation of lymphocytes in CIA mice by activating the Raf/MEK/ERK signal pathway.
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