Abstract: Objective To study the effect of reconstructed bile duct blood flow after arterio-portal shunt on hepatohilar bile duct,liver function and apoptosis of cholangiocytes. Methods Forty-five rats were randomly divided into control group（A）,hepatic artery ligation group（B）,and arterio-portal shunt group（C）,15 rats in each group.Pathological change in hepatohilar bile duct was observed with HE staining,serum ALP and GGT level was measured,and apoptosis of cholangiocytes was detected,3 and 7 days and 1 month after operation,respectively. Results No change was observed in hepatohilar bile duct of group A.Cytoplasmic vacuolization of bile duct epithelial cells occurred 3 days after operation in group B.Hyperplastic reaction was found in bile duct wall with cytoplasmic vacuolization 7 days after operation.The pathological changes disappeared 1 month after operation.No cytoplasmic vacuolization and hyperplastic reaction occurred in bile ducts of group C,with the exception of cellular edema on day 3 after operation.No significant difference was found in serum ALP and GGT level among the 3 groups.No apoptosis of cholangiocytes was observed. Conclusion Arterio-portal shunt can repair the hepatohilar bile duct by ligating the hepatic artery.