异丙酚对肠上皮细胞氧化应激损伤的影响

赵京禹; 郝建华; 李平

异丙酚对肠上皮细胞氧化应激损伤的影响

Effects of propofol on intestinal epithelial cells with oxidative stress injury

摘要

摘要: 目的:探讨异丙酚对大鼠肠上皮细胞(intestinal epithelia cell,IEC)氧化应激损伤的影响。方法:利用H₂O₂产生.OH攻击制备IEC氧化损伤模型。实验设对照组、H₂O₂组(2.5 mmol/L)、异丙酚组(异丙酚100μmol/L)。用四甲基偶氮唑盐(MTT)实验检测IEC的存活率,测定上清测乳酸脱氢酶(LDH)和细胞中丙二醛(MDA)含量。结果:与对照组相比,H₂O₂组LDH、MDA水平明显增高,细胞生存率减少(P<0.01)。异丙酚组与H₂O₂组相比,LDH漏出显著减少,MDA形成减少,并能提高细胞的细胞生存率(P<0.01)。结论:异丙酚对大鼠IEC氧化损伤具有保护作用。

Abstract: Objective:To study the effects of propofol on IEC in the oxidative stress injury induced by H₂O₂.Methods: IEC were cultured in intro,which were treated with H₂O₂,to simulate the IEC injured by OH from H₂O₂.IEC were divided into three group: control group,H₂O₂ group（2.5mmol/L） and propofol group（100μmol/L）.The IEC viability was observed by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay.The lactatc dehydrogenase（LDH） that was relased into the culture media and the malondialdehyde（MDA） content in IEC were measured.Results: Compared with the control group,LDH in the culture media and MDA content in H₂O₂ group were significantly elevated,and the IEC viability obviously decreased（P＜0.01）.LDH in the culture media and MDA content in propofol group were lower than those of H₂O₂ group,and the IEC viability was obviously higher than that of H₂O₂ group.Conclusion: Propofol can protect rat IEC from the oxidative stress injury.

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