壳聚糖温敏凝胶对人牙龈成纤维细胞的毒性研究

Cytotoxicity of chitosan thermosensitive hydrogel to human gingival fibroblasts in vitro

摘要: 目的了解壳聚糖温敏凝胶对人牙龈成纤维细胞的毒性作用,为其治疗牙周病提供依据。方法人牙龈成纤维细胞在不同浓度的壳聚糖温敏凝胶中体外培养24h、48h、72h、96h,用MTT法测定细胞相对增殖率(RGR),判断细胞毒性的级别。结果各组细胞不同时间点相对增殖率均在96%～144%之间,各浓度的壳聚糖水凝胶材料浸提液的细胞毒性均为0级或1级,完全符合生物材料的安全评价标准。结论壳聚糖温敏凝胶对人牙龈成纤维细胞无毒性,用于牙周病局部治疗具有进一步的研究价值。

Abstract: Objective To study the cytotoxicity of chitosan thermosensitive hydrogel to human gingival fibroblasts（HGF）for its application in the treatment of periodontal diseases. Methods Human gingival fibroblasts were cultured in a medium containing chitosan thermosensitive hydrogel at different concentrations for 24,48,72,and 96h,respectively.The relative growth rate（RGR）of HGF was determined by MTT assay. Results The relative growth rate of HGF was over 90%after cultured for different periods of time.The cytotoxicity grade of different concentrations of chitosan thermosensitive hydrogel was 0 and 1. Conclusion RGR have no cytotoxicity to HGF.Local application of chitosan thermosensitive hydrogel in the treatment of periodontal diseases is feasible.