实验性糖尿病大鼠睾丸间质细胞功能和形态变化

The variation of function and morphology of Leydig cells in testis of diabetic rats

摘要: 目的：了解糖尿病对睾丸间质细胞（Leydig细胞）功能和形态的影响。方法：用链脲佐菌素诱导制成糖尿病大鼠模型，2个月后处死大鼠，测定血清睾酮及睾丸组织hCG结合位点，并用光镜和电镜作了睾丸形态学观察。结果：糖尿病大鼠的血清睾酮水平及睾丸组织的hCG结合位点水平明显低于正常对照组（P＜0.01）；糖尿病大鼠Leydig细胞萎缩，内质网、线粒体等细胞器空泡样变。结论：糖尿病可使Leydig细胞功能降低，结构变性。

Abstract: Objective:To investigate the influence of diabeteson the function and morphology of Leydig cells.Methods:The model of diabetic rat was induced by STZ. Two months later, the diabetic rats were sacrificed. Serum testosterone level and hCG binding site of testicular tissue were measured, the morphology of Leydig cells was observed with light and electron microscopy.Results:The serum testosterone level and hCG binding sites of diabetic rats were significantly lower than those of normal rats （P＜0.01）. The morphologocal changes of Leydig cells consisted of cell shrinkage severe mitochondrion and endoplasmic reticulum vacuolization.Conclusions:Diabetes could impaire the function and structure of Leydig cells.