Abstract: Objective: Zn ²⁺ ,Cu ²⁺ ,Ca ²⁺ and Mg ²⁺ ions were determined dynamically in blood and blister fluid in 62 burn patients. Methods: 28 patients had sustained major burn （72.3%±22.7% TBSA with full thickness burns only） were observed. Results: It showed that in malor burn group, blood Zn ²⁺ ,Cu ²⁺ ,Ca ²⁺ and Mg ²⁺ declined significantly on PBD 1. Zn ²⁺ and Cu ²⁺ maintained at low normal levels, but Mg ²⁺ was lower than normal on PBD 1 to PBD 28. There were no significant changes in minor burn group. Meanwhile, the contents of Zn ²⁺ ,Cu ²⁺ ,Ca ²⁺ and Mg ²⁺ were higher in blister fluid and Zn ²⁺ and Ca ²⁺ in blister fluid were markedly higher than those in serum. Conclusion: Exudation from burn wound is an important route for losing of various ions.