Abstract: Objective:To observe the effect of mouse ascites hepatomas cell H22, with characteristic of spontaneous lymphatic metastasis, on proliferation of lymphatic endothelial cell. Methods: Benign lymphangiomas were induced by intro peritoneal injection of incomplete Freund's adjuvant in Balb/c mouse. Lymphatic endothelial cells were isolated from the lymphangioma and cultured in vitro. Mouse ascites hepatomas H22 cells were inoculated in abdominal cavity of Balb/c mouse and generated ascites was harvested to compose conditioned medium（CM）of H22. The proliferation and expressions of flt 4, c fos and PCNA of lymphatic endothelial cell were studied under the effect of different medium. Results: Lymphatic endothelial cells could be isolated from mouse lymphangiomas and grown as confluent or subconfluent monolayer cultures on gelatin coated surface in H22 CM and complete endothelial medium. These cells also positively expressed flt 4, c fos and PCNA. Common medium could neither maintain the proliferation of the isolated cells nor induce expressions of flt 4, c fos and PCNA of the cells. Conclusions: Proliferation of lymphatic endothelial cell might be essential for lymphatic metastasis of cancer cell.