Abstract: Objective To investigate the current disease-related knowledge in chronic hepatitis B （CHB） patients and to analyze its main problems and influencing factors in order to provide the key points of health education and nursing intervention. Methods HBV-related knowledge was investigated in 120 hospitalized CHB patients with questionnaires and analyzed. Results The total standardized score for disease-related knowledge in CHB patients was 7.26±1.57. The score for alcohol abstinence, family protection, and induced factor was 8.92±2.48, 8.33±3.74, and 8.18±2.03, respectively. The score for disease prognosis and infectivity was 4.42±3.09 and 4.94±3.18, respectively. The influencing factors of disease-related knowledge level included culture degree and hospitalization history. Conclusion More attention should be paid to health education of those with a lower education degree and outpatients.