人外周血淋巴细胞体外扩增培养前后CD25、CD29、HLA-DR系列表型研究

张国庆; 焦顺昌; 林星石

人外周血淋巴细胞体外扩增培养前后CD25、CD29、HLA-DR系列表型研究

Phenotypes of CD25,CD29 and HLA-DR cells before and after in vitro amplification from human peripheral blood lymphocytes

摘要

摘要: 目的观察35例恶性肿瘤患者外周血淋巴细胞在体外经扩增培养后细胞CD25、CD29、HLA-DR系列表型的变化。方法体外诱导扩增恶性肿瘤患者外周血淋巴细胞及单个核细胞,应用流式细胞术测定扩增前后CD25⁺、CD29⁺、HLA-DR⁺、CD4⁺CD25⁺、CD4⁺CD29⁺、CD3⁺HLA-DR⁺、CD3⁺HLA-DR^-、CD4⁺HLA-DR⁺、CD4⁺HLA-DR^-、CD8⁺HLA-DR⁺、CD8⁺HLA-DR^-细胞百分比的变化。结果经体外扩增培养后,CD25⁺,CD29⁺,CD3⁺HLA-DR^-,CD8⁺HLA-DR⁺和CD8⁺HLA-DR^-细胞比例较培养前明显增加(P<0.01);而CD4⁺CD25⁺,CD4⁺CD29⁺,CD4⁺HLA-DR⁺和CD4⁺HLA-DR^-细胞比例则明显降低(P<0.01);HLA-DR⁺细胞和CD3⁺HLA-DR⁺细胞培养前后细胞比例变化无统计学意义(P>0.05)。结论经体外扩增培养后,CD4⁺CD25⁺调节性T细胞(Treg)细胞比例降低,活化性T细胞的比例与培养前无统计学差异。

Abstract: Objective To study the phenotypes of CD25,CD29,and HLA-DR cells before and after in vitro amplification from peripheral blood lymphocytes of 35 malignant tumor patients. Methods Lymphocytes and mononuclear cells were induced and amplified from peripheral blood of malignant tumor patients. Percentages of CD25⁺,CD29⁺,HLA-DR⁺,CD4⁺CD25⁺,CD4⁺CD29⁺,CD3⁺HLA-DR⁺,CD3⁺HLA-DR^-,CD4⁺HLA-DR⁺,CD4⁺HLA-DR^-,CD8⁺HLA-DR⁺,and CD8⁺HLA-DR^-cells in effector cells were calculated by flow cytometry,and compared with their percentages before in vitro amplification. Results After amplification,the percentages of CD25⁺,CD29⁺,CD3⁺HLA-DR^-,CD8⁺HLA-DR⁺ and CD8⁺HLA-DR^-cells were significantly increased（P＜0.01）, while the percentages of CD4⁺CD25⁺,CD4⁺CD29⁺,CD4⁺HLA-DR⁺ and CD4⁺HLA-DR^-cells were obviously decreased（P＜0.01）. No significant change was found in the percentages of HLA-DR⁺ and CD3⁺HLA-DR⁺ cells（P＞0.05）. Conclusion After amplification,the percentage of CD4⁺CD25⁺ regulatory T cells（Treg） is obviously decreased while the percentage of active T cells does not change significantly before and after amplification.

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