Abstract: This cell line designated as PLA-802 has been derived from the metastatic tumour tissue in lung and pleura of a patient with laryngeal alveolar rhabdomyosarcoma, 8 hrs after death. It has been transferred for 168 passages in a period of twenty three months.The tumour cells of the cell line consisted of two cytologic types. Cross-striation was demonstrated in the cells by light microscopy. On electron microscopic observation parallel microfilaments with alteried Z band have been found. The growth curve of the cell line was determined from the 56th and 104th passage, of which the cell number was counted daily for a week. The growth curve of the both passages was similar. The doubling time of cell proliferation was 42 hrs. The mode chromosome was 84 and the marker chromosomes in most cells were present in the form of large telomere in the D group.The characteristics of this cell line are rapid proliferation, high malignancy and the properties of myogenic tumour.Other observations such as the Con-A agglutination, planting efficiency, animal transplantation and electron microscopic examination have been briefly discussed.