Abstract: Objective:To investigate whether the total flavonoids of Astragalus（TFA） has an inhibition property on growth of BEL-7402 cells line.Methods:To study the effect of different concentration of TFA for different culture time with BEL-7402 cell line,the 5-FU as a positive control,the changes（such as morphology,curve of survival,curve of growth and the index of karyokinesis） have been assayed.Results:The results showed that the concentration of the half inhibition after treated 48h was（40μg/ml） and 0.3μg/ml for TFA and 5-FU,respectively.The data also indicated that the TFA have a significant property to inhibit BEL-7402 cell growth（P＜0.05） and to reduce the index of karyokinesis when the concentration of TFA was 80μg/ml（（P＜0.05））.Meanwhile,the morphology BEL-7402 have a significant change such as a lot of vacuole in cytoplasm and ambiguous nucleus after treated by TFA for some time.Conclusion:TFA has a potential effect of inhibition BEL-7402 growth.