酶法与苦味酸法测定血清肌酐水平的转换方法
Studies on the conversion method between enzymatic and picric kinetic analysis of serum creatinine
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摘要: 目的: 探索酶法与苦味酸法测定血清肌酐在不同水平段的测定偏差,并建立新旧方法测定结果的转换方法,以满足临床医生的需求。方法: 用两种血清肌酐测定方法同时测定667例来我院门诊就医的不同疾病患者血清肌酐水平,并根据酶法测定值,将其分为5个水平组,统计两种方法的测定数据。结果: 酶法与苦味酸法测定值在各水平组的平均值分别为:A:64.0μmol/L,92.7μmol/L;B:182.5μmol/L,201.9μmol/L;C:378.4μmol/L,384.6μmol/L;D:561.2μmol/L,549.4μmol/L;E:1077.7μmol/L,1025.7μmol/L。两种方法相关均良好,相关系数分别为0.971,0.987,0.950,0.983,0.994。结论: 血清肌酐水平较低时,酶法测定值较与苦味酸法测定值低;在血清肌酐水平为471μmol/L以上时,酶法因方法学优于苦味酸法,而使测定值能够达到真实水平;若已知酶法测定值,临床医生可通过以下公式y(苦味酸法)=0.9186x(酶法)+34.385,得出苦味酸法测定值。反之,则可通过公式y(酶法)=1.0872x(苦味酸法)-37...Abstract: Objective: To evaluate the discrepancy between Creatinine’s enzymatic method and picric kinetic method.Try to found the conversion equation to transfer the results between two methods.Methods:Measure creatinine’s results of 667 patients’ serum with two methods in parallel,then according to enzymatic method,divide the results into 5 level-groups.Averages,standard variances were compared.Results:It showed that all groups had good correlations with two methods（A:0.971,B:0.987,（C:0.950,）D:0.983,E:0.994）.Averages of 5 level-groups were calculated（A:64.0μmol/L,92.7μmol/L;B:182.5μmol/L,（201.9μmol/L）;C:（378.4μmol/L）,384.6μmol/L;D:561.2μmol/L,549.4μmol/L;E:1077.7μmol/L,1025.7μmol/L）.（Conclusion:Creatinine）’sresults with two methods could be switched by equations y（picric method）=0.9186x（enzymatic method）+34.385 and y（enzymatic method）=1.0872x（picric method）-37.065.
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