模拟失重对肺微血管内皮细胞F-actin的影响

Effects of simulated weightlessness on F-actin of pulmonary microvascular endothelial cells

摘要: 目的: 观察回转模拟失重对肺微血管内皮细胞纤维肌动蛋白(F-actin)的影响,探讨其在失重环境下的损伤机制。方法: 组织块贴壁法原代培养肺微血管内皮细胞,采用回转器模拟失重效应。PMVEC回转培养24h、48h和72h,同时设1g同步对照静置培养。采用免疫荧光技术标记PMVEC内的F-actin,以激光共聚焦显微镜观察采集荧光图像。结果: 与同步对照相比,不同时间回转的PMVEC微丝骨架均发生明显破坏,且这种破坏随着回转模拟失重时间的延长而加重。结论: 模拟失重可破坏PMVEC内F-actin,导致PMVEC损伤。

Abstract: Objective: To investigate the effects of simulated microgravity induced by clinostat on filamentous actin（F-action） of pulmonary microvascular endothelial cells（PMVEC） and explore its mechanism. Methods: Clinostat was used to simulate the effects of microgravity.Pulmonary microvascular endothelial cells were cultured on clinostat for 24h,48h and 72h,and then the F-actin of the cells were stained by Texas Red-X phalloidin.The images were collected by laser scanning confocal microscopy（LSCM）. Results: Clinostat induced the depolymerization of F-actin,which increased with the clinostat time. Conclusion: Simulated microgravity can induce the destruction of cytoskeleton by increasing the depolymerization of F-actin and injuring PMVEC.