白色假丝酵母菌基因核型和药敏表型的相关性

雷红; 匡铁吉; 董梅; 佟爱华; 孟祥红; 何菊芳; 朱蕾; 王琰

白色假丝酵母菌基因核型和药敏表型的相关性

Correlation between karyotype and fluconazole susceptibility of Candida Albicans

摘要

摘要: 目的:探讨临床白色假丝酵母菌基因核型和药敏表型的相关性。方法:应用脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)技术分析14株白色假丝酵母菌DNA染色体核型;采用ATB FUNGUS2 INT检测白色假丝酵母菌最低抑菌浓度(MIC);菌种鉴定采用MicroScanWalkAway96 RY-ID快速生化鉴定板。结果:从11例患者的不同标本、不同时期分离出14株白色假丝酵母菌进行基因核型分析,共具有9种不同核型。14株白色假丝酵母菌中有2株对氟康唑耐药(MIC>128μg·mL^-1),其他12株对氟康唑均敏感(MIC<8μg·mL^-1)。结论:在本研究中,基因核型相同或不同的白色假丝酵母菌菌株对氟康唑敏感性不同,表明对氟康唑耐药菌株与敏感菌株基因核型没有显著差异。

Abstract: Objective: To analyze the correlation between karyotype and fluconazole susceptibility of Candida albicans.Methods: 14 isolates of C.albicans were evaluated for molecular relatedness by pulsed-field gel electrophoresis of chromosome-size DNA molecules.Minimal inhibitory concentration（MIC） of fluconazole against C.albicans was determined by ATB FUNGUS2 INT;Isolates were identified by MicroScanWalkAway96 RY-ID.Results: Karyotype revealed a total of 9 different genotypes among 14 isolates of C.albicans from 11 patients.Among the 14 isolates,only two isolates were resistant to fluconazole（＞128 μg·mL^-1）.The rest 12 isolates were all sensitive to fluconazole（＜8 μg·mL^-1）.Conclusion: In our study,there is no distinct difference of karyotype between fluconazole-resistant isolates and fluconazole-sensitive isolates.

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