Abstract: Objective: To detect the prognostic value of the expression of MDM2 and p53 protein, as well as the relationship of these two proteins as determined by immunohistochemistry in bladder cancer. Methods: Bladder tumor samples from 131 patients were assessed immunohistochemically using the specific monoclonal antibody and labeled streptavidin biotin staining against MDM2 and p53 protein. Results: MDM2 and p53 positive stains were 39 cases （29.8%） and 87 cases （65.6%）, respectively There was no significant correlation of MDM2 staining with the histological grade, clinical stage and prognoses p53 accumulation were significantly correlated with high histologic grade （P=0.0012）, high clinical stage （P＜0.0001） and reduced survival （P=0.007） No relationship was observed between MDM2 and p53 in staining status or intensity. The lowest survival rate was seen in p53+/MDM2, pattern Multivariate analysis demonstrated that p53⁺/MDM2 has significant statistical correlation with unfavorable outcome （P=0.0046） Conclusion: p53+/MDM2 pattern is independent prognostic impact for tumor progression in bladder cancer. The simultaneous immunostaining for MDM2 and p53 could be important prognostic factor in patients affected by bladder cancer.