银杏提取物对鼠脊髓损伤后神经细胞的保护及功能恢复作用
Protective effect and recovery function of ginkgo biloba extract on rats on spinal cord injury
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摘要: 目的: 研究银杏提取物(GBE)对大鼠脊髓损伤后神经细胞的保护及功能恢复作用。方法: 75只W istar雄性大鼠,随机分为假手术组(A),对照组(B)和治疗组(C),每组25只,A组只打开椎板,不损伤脊髓,B组脊髓损伤后腹腔注射等渗盐水(NS),C组脊髓损伤后1 h腹腔注射GBE 40 mg/kg,此后每天1次,持续治疗5 d。末次治疗后每组处死5只动物,检测脊髓组织SOD活性,MDA和NO含量,脊髓损伤后第1 h和末次治疗后第1天、第5天、第10天评定后肢运动功能(Salzm an评分)和检测MEP。结果: SOD活性C组高于B组(P<0.05),MDA和NO含量C组低于B组(P<0.05)。脊髓损伤后,后肢运动障碍,MEP波幅(P1-N1峰峰值)降低,潜伏期延长,末次治疗后各时间,治疗组MEP波幅、潜伏期,神经功能恢复均优于对照组(P<0.05)。结论: 银杏提取物能减少脊髓损伤后氧自由基所致继发损伤,并促进神经功能恢复。Abstract: Objective: To study the protective effects and recovery function of Ginkgo biloba extract(GBE) on rats spinal cord injury. Methods: 75 wistar male rats were randomly divided into 3 groups: A(sham-operated group);B(control group) C(GBE treatment group).group B treatel with NS and group C,GBE 40mg/kg,were respectively intraperitoneally injected into the rats 1 hour after SCI,then given GBE at a day interal 5 deys.After the last treatment,the 5 rats(each group) were killed and the levels of SOD、MdA and NO were measured and after last treatment 1、 5、 10 days motor function of lowor limb was evaluated according to Salzma’s grading eriteria and MEP was recorded. Results: The levels of SOD in group C was Singnificantly higher than in group B(P<0.05) and the level of MDA、NO in group C was Significantly lower than in group B(P<0.05).Distinct handicap of motor function in lower limbs presented and amplitudes(Peak-Peak between P1 and N1) of MEP reduced,Amplitudes in group C recovered better than in group B in each measured time(P<0.05). Conclusion: GBE can decrease the production of free radicals after SCI and accelerate undee reversion of MEP and promote motor function recovery of SCI.undee restoration of MEP begans ealier than that of motor function of lower limbs.
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