皮肤低温损伤机制中海藻糖的抗氧自由基作用

Trehalose’s protective effects of cryopreserved skin to resist the damage of oxygeon free radical

摘要: 目的: 观察海藻糖在低温储存皮肤组织中的抗氧自由基损伤作用。方法: 取新鲜皮肤标本做正常对照组,另行海藻糖和二甲基亚砜处理后液氮低温储存,复温后测定皮肤组织匀浆液中超氧化物歧化酶(SOD)活力,过氧化脂质(LPO)代谢产物丙二醛(MDA)含量。结果: 经海藻糖处理后,低温储存的皮肤组织中SOD活力升高,其MDA含量下降。结论: 海藻糖是一种有效的稳定剂,在皮肤低温损伤中具有抗氧自由基损伤作用。

Abstract: Objective: To investigate the protective effects of trehalse during cryoinjury.Methods: Evaluate the level of superoxide dismutaes（SOD） and the amount of malonyldialdehyde（MDA） from the cryopreserved skin.Results: Comparing to the normal human skin, trehalose could enhance the vitality of SOD and decrease the production of MDA when the skin was frozen.（Conclusion: ）It showed that trehalose may promote the ability on elimination of oxygen free radical and decrease the attack of the oxygeon free radical during cryoinjury.trehalose;oxygeon;free radical;hypothermia;skin