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全氟化碳雾化吸入对大鼠海水吸入性肺损伤治疗作用研究

程志鹏 刘金霞 苏程程 梁志欣

程志鹏, 刘金霞, 苏程程, 梁志欣. 全氟化碳雾化吸入对大鼠海水吸入性肺损伤治疗作用研究[J]. 解放军医学院学报, 2023, 44(5): 508-513. doi: 10.3969/j.issn.2095-5227.2023.05.012
引用本文: 程志鹏, 刘金霞, 苏程程, 梁志欣. 全氟化碳雾化吸入对大鼠海水吸入性肺损伤治疗作用研究[J]. 解放军医学院学报, 2023, 44(5): 508-513. doi: 10.3969/j.issn.2095-5227.2023.05.012
CHENG Zhipeng, LIU Jinxia, SU Chengcheng, LIANG Zhixin. Therapeutic effect of perfluorocarbon aerosol inhalation on lung injury induced by seawater inhalation in rats[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2023, 44(5): 508-513. doi: 10.3969/j.issn.2095-5227.2023.05.012
Citation: CHENG Zhipeng, LIU Jinxia, SU Chengcheng, LIANG Zhixin. Therapeutic effect of perfluorocarbon aerosol inhalation on lung injury induced by seawater inhalation in rats[J]. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2023, 44(5): 508-513. doi: 10.3969/j.issn.2095-5227.2023.05.012

全氟化碳雾化吸入对大鼠海水吸入性肺损伤治疗作用研究

doi: 10.3969/j.issn.2095-5227.2023.05.012
基金项目: 基础加强计划重点基础研究项目(2021-JCJQ-ZD-077-12)
详细信息
    作者简介:

    程志鹏,男,在读硕士。Email: chengzhipeng301@163.com

    通讯作者:

    梁志欣,男,博士,主任医师,教授。Email: liangzhixin301@163.com

  • 中图分类号: R568.3

Therapeutic effect of perfluorocarbon aerosol inhalation on lung injury induced by seawater inhalation in rats

More Information
  • 摘要:   背景  海水吸入性肺损伤是呼吸系统危重症,致死率高,目前缺乏有效治疗方法。全氟化碳(perfluorocarbon,PFC)是一种无色无味呈惰性的有机化合物,在多种急性肺损伤动物模型中表现出抗炎、抗氧化、减轻肺损伤等作用,目前无PFC雾化吸入对海水淹溺导致的急性肺损伤干预作用的研究。  目的  本研究拟探讨PFC雾化吸入对海水吸入性肺损伤的治疗作用。  方法  32只雄性SD大鼠随机分为4组,分别为0.9%氯化钠注射液组(N组)、全氟化碳雾化吸入组(P组)、海水吸入 + 0.9%氯化钠注射液吸入组(SW组)、海水吸入 + PFC雾化吸入组(SP组),每组8只。SW组和SP组在建立海水吸入性肺损伤模型后30 min,分别经气管给予0.9%氯化钠注射液(2 mL/kg)和PFC (2 mL/kg)雾化吸入。于建模后4 h取肺组织、外周血和肺泡灌洗液,检测组织病理学、炎症指标[肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-1β、IL-6]、氧化应激指标[髓过氧化物酶(myeloperoxidase,MPO)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)]、肺湿干比、肺泡灌洗液蛋白浓度,取右下肺做HE染色。另取16只雄性SD大鼠,分为4组,每组4只(各组处理方式同上),各组于处死前30 min经右侧股静脉注射2%伊文斯蓝染料(20 mg/kg),测定肺组织伊文斯蓝含量。  结果  与N组相比,SW组肺损伤较重,肺损伤病理评分较高(P<0.05),血清及肺泡灌洗液TNF-α、IL-1β、IL-6、MDA、MPO、肺组织湿干比、肺泡灌洗液蛋白含量及肺组织伊文斯蓝水平显著升高(P<0.05),SOD含量降低(P<0.05)。海水吸入后给予PFC雾化吸入,可显著降低肺损伤病理评分、血清及肺泡灌洗液TNF-α、IL-1β含量、肺泡灌洗液IL-6、血清MDA、蛋白含量、湿干比、肺组织伊文斯蓝含量(P<0.05),SOD含量显著升高(P<0.05),而血清IL-6、MPO在SW组与SP组间无统计学差异。  结论  全氟化碳雾化吸入可显著减轻大鼠海水吸入导致的肺损伤,其机制可能与改善肺内炎症反应、氧化应激和肺泡毛细血管通透性有关。

     

  • 图  1  大鼠肺部微液体量雾化针及雾化效果图

    Figure  1.  MicroSprayer aerosolizer and its effect

    图  2  各组大鼠血清和肺泡灌洗液中TNF-α、IL-1β、IL-6浓度

    Figure  2.  Concentration of TNF-α, IL-1β and IL-6 in BALF of the rats in each group

    图  3  各组大鼠血清MPO、MDA、SOD浓度

    Figure  3.  Sera MPO, MDA and SOD concentration of the rats in each group

    图  4  各组大鼠肺组织HE染色病理结果(200×)

    Figure  4.  HE staining pathological results of lung tissues of rats in each group (200×)

    图  5  各组大鼠海水吸入性肺损伤病理评分

    Figure  5.  Pathological score of seawater inhalation lung injury in the rats of each group

    图  6  各组大鼠肺组织湿干比和肺泡灌洗液蛋白含量

    Figure  6.  Wet-to-dry ratio and alveolar lavage fluid protein content of lung tissues in the rats of each group

    图  8  各组肺组织伊文思蓝含量

    Figure  8.  Evans blue content level in lung tissues of each group

    图  7  各组大鼠肺组织伊文思蓝染色后肺标本

    Figure  7.  Lung specimens after Evans blue staining of lung tissues in the rats of each group

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出版历程
  • 收稿日期:  2022-12-09
  • 网络出版日期:  2023-04-14
  • 刊出日期:  2023-05-28

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