大鼠完全肠外营养时胆汁中8种游离脂肪酸的改变

Changes of eight free fat acids in bile of rats during total parenteral nutrition

摘要: 目的：研究完全胃肠外营养（TPN）支持时胆汁中游离脂肪酸（FFA）的变化。方法：采用气相色谱法测定胆汁游离脂肪酸。结果：不含脂肪乳TPN组大鼠胆汁十四碳酸（C_14∶0）、软脂酸（C_16∶0）、硬脂酸（（C_18∶0）及花生四烯酸（C20∶4）浓度构成明显高于对照组及含脂TPN组，而亚油酸（C_18∶2）、亚麻酸（C18∶3）下降；含脂TPN组胆汁中除油酸（C_18∶1）含量高于对照组外，余无异常。结论：含脂肪乳剂的TPN可以减轻大鼠胆汁脂肪酸的异常变化，对预防TPN时的胆道并发症将有重要意义。

Abstract: Objective:To investigate the changes of 8 free fatty acids（FFA） in bile of rats during total parenteral nutrition（TPN）.Methods: Measuring the FFAs in bile by gas chromotography Results:（1）Bile percentage concentrations of FFAs of C_14∶0,C_16∶0,C_18∶0,and C_20∶4,in fat-free TPN group were remarkably higher than that of controls and lipid-TPN groups, but with decreased C_18∶2 and C_18∶3,in fat-free TPN group; （2）No difference was found between controls and lipid-TPN groups in percentage concentrations of FFA in bile except that C_18∶1 was higher than that of controls.Conclusion:Lipid-TPN may reduce the degree of bile FFA change during TPN, and thus play an important role in the prevention of TPN-indued biliary complications.