急性和慢性白血病Livin基因表达的研究
Expression of Livin gene in acute leukemia and chronic myelocytic leukemia
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摘要: 目的: 研究Livin基因在急、慢性白血病中的表达,探讨其临床意义。方法: 采用半定量逆转录-聚合酶链反应(RT-PCR)检测91例急性白血病(AL)和17例慢性粒细胞性白血病(CML)患者Livin mRNA的表达水平,21名健康人的标本作正常对照。结果: 初治AL患者中Livin的表达较正常对照明显升高,治疗缓解后的表达水平下降,复发时又再次升高;慢性粒细胞白血病慢性期(CML-CP)、加速/急变期(CML-AP/BP)患者Livin的表达水平均高于正常对照组;初治AL患者中,Livin阳性的患者缓解率低于表达阴性的患者。结论: Livin基因的过度表达参与了急、慢性白血病的发病,并且是AL复发的高危因素;Livin基因的高表达是判断急性白血病预后不良的指标之一。Abstract: Objective: To investigate the expression of Livin in leukemia and the prognostic significance in acute leukemia（AL） in adults. Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia（CML） patients by semi-quantity reverse transcription polymers chain reaction（RT-PCR）.21 healthy adults were also measured as normal control. Results: The expression of Livin in de novo AL was higher than that in normal control,while it decreased in patients with complete remission.In relapsed patients,the expression of Livin increased again.The level of Livin mRNA in CML was significantly higher than that in normal controls.In AL patients,the CR rate in Livin+ cases was lower than that in Livin cases.Conclusions: Overexpression of Livin may play a synergic role in the pathogenesis of leukemia and may be a high risk factor for relapse in AL.High expression of Livin can serve as one of the markers of poor prognosis in AL.
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