Abstract: Objective To establish the platform for evaluating the effect of gunpowder smoke on cultured bronchial epithelial cells. Methods A gas generating device was designed for the interaction between gas and cells.Cultured human bronchial epithelial cells were directly contacted with the gunpowder smoke in its original state.Different amounts of gunpowder smoke and treatment time were used as an indicator to observe the effect of gas in the device on the activity and metabolism of bronchial epithelial cells. Results The gas generating device could induce bronchial epithelial cell injury in a dose-and time-dependent manner while the device itself had no effect on the cells,suggesting that the original gas in the smoke generating device can directly interact with the cells and the device can be used in analysis of gunpowder inhalation toxicology. Conclusion The experimental device,simple in structure and low in cost,can detect the toxicological effect of gas on the activity and metabolism of bronchial epithelial cells at cellular level,reduce the use of laboratory animals,and can thus be widely used in toxicological analysis of gas.