羊蛛网膜下植入APA微囊化牛嗜铬细胞脑脊液亮氨酸脑啡肽和儿茶酚胺含量的变化

郭水龙; 薛毅珑; 崔忻; 李新建; 罗芸; 何立敏

羊蛛网膜下植入APA微囊化牛嗜铬细胞脑脊液亮氨酸脑啡肽和儿茶酚胺含量的变化

Concentration change of catecholamine and leucine-enkephalin in sheep cerebrospinal fluid after APA microencapsulated bovine chromaffin cells transplantation to the spinal subarachnoid space

摘要

摘要: 目的: 了解APA微囊化牛嗜铬细胞(APA-BCCs)蛛网膜下植入引起正常羊脑脊液中儿茶酚胺(cate cholamine,CA)和亮氨酸脑啡肽(leucine enkephalin,L-EK)含量的变化,分析APA-BCCs移植镇痛的可能机制及APA微囊的免疫保护效应。方法: 以手术方法分别将APA-BCCs、BCCs或空微囊植入正常羊脊髓蛛网膜下,测定移植前后不同时间羊脑脊液中去甲肾上腺素(norepinephrine,NE)、肾上腺素(epinephrine,E)和L-EK的含量。结果: 与移植前羊脑脊液中NE、E和L-EK的水平相比,空微囊植入未能升高;BCCs植入可在7d内升高;APA-BCCs植入可明显升高超过2 8d (P<0.05)。结论: 蛛网膜下植入BCCs能显著升高宿主羊脑脊液中CA和L-EK的水平,这可能与BCCs蛛网膜下移植的镇痛效应有关;APA微囊包裹有利于BCCs在异种动物体内较长期地分泌镇痛物质。

Abstract: Objective: To observe the change of catecholamine （CA） and leucine-enkephalin（L-EK） concentration in sheep cerebrospinal fluid after APA Microencapsulated Bovine Chromaffin Cells transplantation to the spinal subarachnoid space, to analyze the analgesic mechanism of transplantation and the immuno-protecting effect of APA-microcapsulation. Methods: To check concentration of norepinephrine（NE）, epinephrine（E） and leucine-enkephalin（L-EK） in sheep cerebrospinal fluid after APA Microencapsulated Bovine Chromaffin Cells（APA-BCCs）, BCCs and hollow microcapsule transplantation to the spinal subarachnoid space. Results: the concentration of E, NE and L-EK didn’t increased after transplantation of hollow microcapsule,increased after BCCs transplantation within 7 days and APA-BCCs transplantation within 28 days. Conclusion: BCCs transplantation to the spinal subarachnoid space could improve the concentration of CA and L-EK in host cerebrospinal fluid, the improvement may be related to analgesic effect, and APA-microencapsulation is good for lasting secretion of BCCs.

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