Abstract: Objective To develop the non-transgenic method for inducing transcription of oct4 gene in epidermic stem cells. Methods RT-PCR was performed to measure the transcription level of oct4 gene in epidermic stem cells 48h after activated by 6-aminohexanoic acid. Results 6-aminohexanoic acid effectively activated the transcription of oct4 in epidermic stem cells. Conclusion RT-PCR can accurately and repeatedly detect the transcription level of oct4 gene in epidermic stem cells,and is thus a simple and practical method for screening the potential small molecular compounds expressed in inducing transcription genes.