Abstract: Objective: To study the efficient、fast and simple method to be used in the carrier detections of DMD/BMD. Methods: We developed the method of duplex quantitative PCR approach and DQ value to detecte deletion carriers of DMD/BMD. Parts of the results were compared with Short Tandem Repeat sequence PCR （STRs PCR） method and CK value. Results: We determined the normal and abnormal reference value with analyses of 7 obligated deletion carriers, 21 possible carriers and 26 normal female controls, and found entire coordinate in 8 female relatives whose status had been determined by STRs analysis. It has been shown that quantitative PCR is more sensitive than that of CK value. Conclusion: The quantitative duplex PCR assay is suitable for routine application.