Abstract: Objective: To analysis drug resistant of pseudomonas aeruginosa in the elder patients with tracheal incision of different time. Methods: 158strains drug sensitivity tests of pseudomonas aeruginosa in the elder patients with59cases tracheal incision in half year,56strains drug sensitivity tests of pseudomonas aeruginosa in the elder patients with16cases tracheal incision from half year to one year and 57strains drug sensitivity tests of pseudomonas aeruginosa in the elder patients with13cases tracheal incision over one year were compared. Results: There were no statistical changes of the drug-resistant rates of pseudomonas aeruginosa in the elder patients with tracheal incision of different time;More than 50% drug-resistant rates of pseudomonas aeruginosa were ampicillin,aztreonam,carbenicillin,cefazolin,cefotaxime,ceftizoxime,ceftriaxiome,cefuroxime, cephalothin,ciprofloxacin,meziocillin,ticarcillin,timentin,cefoperazone.Less than50% drug-resistant rates of pseudomonas aeruginosa were amikacin,ceftazidime,gentamicin,impenem,piperacillin. Conclusion: There were no statistical changes of the drug resistant of pseudomonas aeruginosa in the elder patients with tracheal incision of different time;drug-resistant of pseudomonas aeruginosa for most antibiotics were severely in the older patients with tracheal incision;Impenem,piperacillin, ceftazidime were more sensitivity for pseudomonas aeruginosa in the elder patients with tracheal incision.