Abstract: Objective To investigate the protective effects of electro-acupuncture （EA） at Zusanli point on myocardial injury induced by proinflammatory factors in rats. Methods Thirty-two male SD rats were selected to establish a sepsis model of rats by cecal ligation and puncture （CLP）, and randomly divided into CLP+ EA group, CLP+ sham EA（SEA） group, vagotomy（VA）+CLP group, and VA+CLP+EA（VA/EA） group （n=8）. EA was performed at bilateral Zusanli points （ST36） of rats in EA group with a constant voltage （2mA, 2-100Hz） for 1h after CLP. SEA was performed in rats of VA and VA/EA groups. Rats in EA group were sacrificed 6h after CLP, and myocardial specimens were harvested for evaluation of tumor necrosis factor（TNF-α）, nitric oxide（NO）, myeloperoxidase（MPO） and assessment of the rate of water content （ratio of dry to wet weight）. Plasma activity of creatine kinase MB isoenzyme （CK-MB） was also determined. Results The levels of TNF-α, NO, MPO, the rate of water content in myocardium, the plasma activity of CK-MB were significantly lower in EA group than in other three groups 6h after CLP（P＜0.05）. The levels of TNF-α, NO, MPO, the rates of water content were significantly higher in VA and VA/EA groups than in SEA group（P＜0.05）. There was no statistical difference in above measurements between VA and VA/EA groups. Conclusion EA at Zusanli points can decrease the levels of proinflammatory factors 6h after CLP, and VA can weaken or eliminate the effects of EA, and its mechanism may be related with the cholinergic anti-inflammatory pathway.