Abstract: Objective To evaluate the therapeutic effect of combined doxycycline and interferon suppository or doxycycline alone on female genital tract infection. Methods Seventy-six patients infected with ureaplasma unrealytium and mycoplasma hominis who visited our department from October 2009 to October 2010 were randomly divided into combined drug treatment group（group A） and single drug treatment group（group B）.Vulvovagina of patients in group A was irrigated with 0.9% normal saline and 5% iodophor（1:10）.Then,the patients were treated with doxycycline（4mg）,once a day for 7 days.Interferon suppository was placed in the deep vagina,every other day for 14 days.Patients in group B were treated with oral doxycycline only（4mg）,once a day for 7 days.One week after withdrawal of the drugs,the patients in two groups underwent gynecological examination.Two weeks after withdrawal of the drugs,mycoplasma from cervix was cultured. Results The sensitivity rate of the 76 patients to doxycycline,josamycine and minocycline was 61.9%,59.2% and 50%,respectively.Their drug resistance rate to roxithromycin,spiromycin,Clarithromycin was 90.8%,88.2% and 76.3%,respectively.The total effective rate for groups A and B was 92.1% and 78.9%,respectively（P＜0.001）. Conclusion The therapeutic effect of combined doxycycline and interferon suppository is better than doxycycline only on ureaplasma urealyticum accompanying mycoplasma hominis infection in female genital tract.