EGFR基因在肺腺癌中突变的研究

Epidermal growth factor receptor mutation in lung adenocarcinoma

摘要: 目的:探讨表皮生长因子受体(EGFR)基因酪氨酸激酶域体细胞在肺腺癌患者中突变的相关因素。方法:分析和检测64例肺腺癌石蜡包埋标本EGFR基因突变状况,采用PCR和序列分析技术进行EGFR基因18、19和21外显子突变分析。结果:64例PAC患者中有16例(25%)酪氨酸激酶域存在体细胞突变。其中2例(3.1%)为18外显子,7例(10.93%)为19外显子,7例(10.93%)为21外显子。结论:肺腺癌(PAC)患者肿瘤组织中EGFR基因突变主要集中于19、21外显子突变,女性高于男性。

Abstract: Objective: To study Somatic mutation of the tyrosine kinase（TK） domain of the epidermal growth factor receptor（EGFR gene） in lung adenocarcinoma patients.Methods: Mutational analysis for EGFR exons 18,19,20 and 21 was performed in 64 patients.Results: Somatic mutations in TK domain of EGFR were identified in 16 of the 64（25%） patients,including 2 cases in exon 18（3.12%）,7 cases in exon 19（10.93%） and 7 cases of in exon 21（10.93%）.Conclusion: EGFR gene mutations is concentrate on 19 and 21 hot sport.The mutation rate of women is higher than that of men.