雌激素受体α与其靶基因LRP16相互作用的研究

Study on the interaction of estrogen receptor α and its target gene LRP16

摘要: 目的:既往研究表明LRP16是雌激素受体α(ERα)诱导表达的一个靶基因,但可以反馈激活ERα介导的转录活性,本研究目的在于探讨该反馈效应的分子机制。方法:GST-pulldown与免疫共沉淀(CoIP)方法检测LRP16与ERα体内外的相互作用。结果:GST-pulldown与CoIP实验结果均表明LRP16与ERα可以直接结合,并且表明该相互作用不依赖于雌激素的存在,但雌激素可以增强二者的结合。结论:LRP16不仅是ERα的一个下游靶基因,而且是ERα的一个共激活因子。

Abstract: Objective:LRP16 is an estrogen receptor（ERα） target gene,and the ERα-mediated transactivation is enhanced by LRP16.The study is to investigate the molecular mechanism underling this feedback effect.Methods: The in vitro and in vivo interactions of ERα and LRP16 were detected by GST-pulldown and coimmunoprecipitation（CoIP） assays,respectively.Results: LRP16 could directly bind to ERα in an estrogen-independent manner,but could be enhanced by estrogen.Conclusion: LRP16 is not only an estrogen-induced gene by ERα,but also acts as a coactivator of ERα.