纳洛酮对缺氧-复氧心肌细胞线粒体能量代谢的保护作用
Effect of Naloxone on delay of the collapse of mitochondrial membrane potential and improvement of energy metabolism of cultured myocardial cell induced by hypoxia and reoxygenation
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摘要: 目的: 观察纳洛酮对培养家兔心室肌细胞缺氧-复氧后线粒体膜电位变化的影响,探讨其对缺氧心肌细胞能量代谢的保护意义。方法: 培养心室肌细胞分成三组: 对照组(A组)、缺氧组(B组)、纳络酮组(C组),后两组又分3个亚组: 缺氧2 h组(B1、C1);缺氧2 h-复氧2 h组(B2、C2);缺氧2 h-复氧4 h组(B3、C3)。检测缺氧-复氧后不同时间细胞线粒体膜电位变化情况。结果: 缺氧后,线粒体膜电位均下降;C组各时间点线粒体膜电位均高于B组(P≤0.01)。结论: 早期应用纳洛酮可明显减轻心室肌细胞缺氧-复氧后线粒体膜电位的下降,改善缺氧心肌细胞的能量代谢。Abstract: Objective: To study the effect of Nalonxe on mitochondrial membrane potential（MMP） in cultured myocardial cell after hypoxia-reoxygenation and its possible protection to cardialmyocytes′energy metabolism.Methods: cultured cardiomyocytes from neonate rabbits were divided into three groups（control group,hypoxia group and naloxone group） and the two latter were further divided into three subsections: hypoxia 2 hour,hypoxia 2 h-reoxygen 2 h,hypoxia 2 h-reoxygen 4 h.Then the cells were stained with fluorescent dyes and MMP was assayed by flow cytometry.Results: After hypoxia,MMP of hypoxia group and Naloxone group were lower than the control（P≤0.01）.MMP of therapy group was significantly higher than that of hypoxia group on each different time point（P≤0.01）.Conclusion: Naloxone might significantly reduce the lossing of MMP of hypoxia-reoxygenation injured cardialmyocytes and improve their energy metabolism.
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