Abstract: Objective: To study the effect of peritoneal macrophages（PM） on peritonitis of rats with experimental pancreatitis. Methods: A model of hemorrhagic necrotic pancreatitis was established by retrograde injection of 2% sodium deoxycholate into the common biliopancreatic duct of rats.Liposome-CL2MBP was injected into the peritoneum to exhaust PM of rats.Twenty-four male rats were randomly divided into PMs（+） group,PMs（-） group and sham operation group.The rats were killed 12h after the model was established to take samples.Pancreatic pathology was examined.Ascite lavage was quantified.Serum AMY,cytokine and NO levels were measured. Results: No significant difference was found in pancreatic pathology and serum AMY level between PMs（+） and PMs（-） groups,but a significant difference was observed in ascite lavage quantity and serum cytokine and NO level between the two groups. Conclusion: PM have important effects on peritonitis of rats with acute pancreatitis.