十一酸睾酮对胃上皮细胞生长的实验研究

黄海力; 吴本俨; 徐世平; 李小鹰

十一酸睾酮对胃上皮细胞生长的实验研究

The effects of testosterone undecanoate on human gastric cell HFI-145 growth in vitro

摘要

摘要: 目的：观察十一酸睾酮对正常胃上皮细胞株HFI-145生物学行为的影响，评价此药的安全性。方法：用不同浓度的十一酸睾酮作用细胞，MTT方法研究细胞生长状况，FCM观察细胞周期分布，TUNEL法检测细胞凋亡。比较不同浓度作用组间差异以及与对照组的差异。结果：0.0004μmo/l L～0.4μmo/l L十一酸睾酮对HFI-145细胞作用不明显，4μmo/l L浓度能抑制细胞生长，并且有时间依赖性，作用第3天抑制率为27%；4μmo/l L用药组细胞的G0/G1期比例较对照组增多；FCM以及TUNEL未发现凋亡百分比的改变。结论：药理浓度范围内十一酸睾酮对正常胃粘膜上皮细胞无毒性作用，高浓度十一酸睾酮抑制胃上皮细胞生长。

Abstract: Objective：to investigate the effects of the anbolic-androgenic steroids testosterone undecanoate（TU）on the growth of human gastric cell HFI-145.Methods：MTT colorimetric assay was used to measure the pfoliferation of HFI-145cells exposed to different concentration of TU.Apoptosis and cell cycle phase distribution were measured by TUNEL and flow cytometric analysis between the 4μmo/l L TU treated group and nontreated group.Results：Compared with nontreated group，TU from 0.0004 to 0.4μmo/l L exerted no action on HFI-145cell.High concentration of TU（4μmo/l L） had an inhibitory effect on the proliferation of HFI-145cells by induced HFI-145to G0/G1arrest on a time-depending manner which peaked at 72h.The maximum inhibitory rate was 27%.Conclusion：In vitro，high concentration of TU inhibits the growth of human nomal gastric cells by arresting the cell cycle progression in G0/G1phase while pharmacological concentration of TU has no effects on HFI-cell growth.

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