Abstract: Objective To study clinical value of uniportal video-assisted thoracoscopic surgery（VATS） for primary lung cancer. Methods VATS was performed for primary lung cancer in 29 patients with lobectomy and dissection of systematic mediastinal lymph nodes from September 2009 to March 2010 in our department. Results Of the 29 patients,11 underwent right upper lobectomy,5 right middle lobectomy,7 right lower lobectomy,1 left upper lobectomy,5 left lower lobectomy.The procedure was successful in all patients with no patient transferred for thoracotomy.The mean operative time was 150.7±36.8min,the average number of dissected lymph nodes from each patient was 12.6.The lung cancer was classified as TNM stageⅠa,Ⅰb,Ⅱa,Ⅱb,andⅢa in 13,9,2,1,and 4 patients,respectively,accounting for 44.8%,31.0%,6.9%,3.4%,and 13.8% of the total patients,respectively.All patients recovered uneventfully with no severe complications such as pulmonary infection,arrhythmia,and bleeding.No postoperative death occurred. Conclusion Uniportal VATS is technically feasible for lung cancer and can complete lobectomy and dissection of systematic mediastinal lymph nodes with the advantages of minimal invasiveness and rapid postoperative recovery.