人外周血淋巴细胞体外扩增培养前后19种细胞表型研究

张国庆; 焦顺昌; 林星石

人外周血淋巴细胞体外扩增培养前后19种细胞表型研究

Study on 19 kinds of lymphocyte phenotype before and after in vitro amplified from human peripheral blood lymphocyte

摘要

摘要: 目的:观察35例恶性肿瘤患者外周血淋巴细胞在体外经扩增培养后19种细胞表型的变化。方法:体外诱导扩增恶性肿瘤患者外周血淋巴细胞及单个核细胞,应用流式细胞术测定扩增前后CD3⁺等19种不同表型细胞百分比的变化。结果:经体外扩增培养后,CD3⁺、CD3⁺CD8⁺、CD3⁺CD16-CD56⁺、CD3⁺CD16⁺CD56^-、CD3⁺CD16⁺CD56⁺,CD45RO⁺细胞及其亚型CD8⁺CD45RO⁺、CD8⁺CD28^-细胞比例较培养前明显增加(P<0.01);而CD19⁺、CD3⁺CD4⁺、CD3^-CD16^-CD56⁺、CD3^-CD16⁺CD56^-、CD3^-CD16⁺CD56⁺,CD45RA⁺细胞及其亚型CD4⁺CD45RA⁺、CD8⁺CD45RA⁺,CD4⁺CD45RO⁺,CD28⁺细胞及其亚型CD8⁺CD28⁺细胞比例则明显的降低(P<0.01)。结论:培养扩增后细胞具有直接杀伤肿瘤细胞的细胞毒活性。

Abstract: Objective: To study the 19 kinds of lymphocyte phenotype before and after in vitro amplified from 35 malignant tumor patients’ peripheral blood lymphocyte.Methods: The lymphocyte cells and mononuclear cells from peripheral blood of malignant tumor patients were induced and amplified,and the flow cytometry was used to detect the percentage of CD3⁺,etc.19 type cells in effector cells,and then their percentages before and after the in vitro amplification were compared.Results: After amplification,the percentage of CD3⁺,CD3⁺CD8⁺,CD3⁺CD16^-CD56⁺,CD3⁺CD16⁺CD56^-,CD3⁺CD16⁺CD56⁺,CD45RO⁺ and it's subgroups CD8⁺CD45RO⁺,CD8⁺CD28^-cells significantly increased（P＜0.01）;the percentage of CD19⁺,CD3⁺CD4⁺,CD3^-CD16^-CD56⁺,CD3^-CD16⁺CD56^-,CD3^-CD16⁺CD56⁺,CD45RA⁺ and it's subgroups CD4⁺CD45RA⁺,CD8⁺CD45RA⁺,CD4⁺CD45RO⁺,CD28⁺ and it's subgroup CD8⁺CD28⁺ cells significantly decreased（P＜0.01）.Conclusion: After amplification,the effector cells have the cytotoxic activity that can directly kill the tumor cells.

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