Abstract: Objective To observe the effect of +Gz on cholecystokinin（CCK）,somatostatin（SS）,biliary tract pressure,and bile duct flow in rabbits on high cholesterol diets. Methods Thirty male rabbits were randomly divided into 2-,4-and 6-week high cholesterol groups（control groups）,high cholesterol plus 2-,4-and 6-week +Gz groups（experimental groups）,5 rabbits in each group.Their CCK,SS,biliary tract pressure,and bile duct flow were measured. Results The CCK level was lower in 3 experimental groups than in 3 control groups（F=85.845,P＜0.01）,which decreased with +Gz exposure time,while the SS level was significantly higher in 3 experimental groups than in 3 control groups（F=71.081,P＜0.01）,which increased with the +Gz exposure time.The biliary tract pressure was higher in 3 experimental groups than in 3 control groups（P＜0.05）,which decreased with the +Gz exposure time.The bile duct flow rate was lower in 3 experimental groups than in 3 control groups（F=28.354,P＜0.05）,which decreased with the +Gz exposure time. Conclusion +Gz exposure may decrease the CCK level,increase the SS level,lead to abnormal bile cyst empting function and bile duct pressure in rabbits on high cholesterol diets,suggesting that +Gz exposure can accelerate the formation of gallstones.