ID4基因表达谱的生物信息学分析
Analysis of ID4 gene expression series with bioinformatics
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摘要: 目的: 分析ID4基因在正常组织细胞和癌变组织细胞中的表达情况,为研究该基因的表达调控机制奠定基础。方法: 采用生物信息学方法,利用开放性的数据库包括Gene expression omnibus(GEO)、Affymetrix基因芯片表达数据库、人类基因组数据库(GeneBank)、基因表达数据库SAGE、GeneCard、InterPro、ProtoNet、UniProt和BLOCKS。结果: ID4基因在多种神经系统肿瘤细胞中的表达高于正常脑组织(209/3,148/3,123/3,72/3,64/3,45/3);而甲状腺滤泡癌的表达要低于正常甲状腺组织(35/172);正常乳腺组织要明显低于转移乳腺癌和原发乳腺癌的表达(10/36,10/34);正常肺组织的表达高于肺腺癌(11/4)。此外,ID4基因在正常淋巴结、胚胎干细胞等细胞中也有表达。结论: 生物信息学可以用来方便快捷地分析基因的表达谱;ID4基因的表达具有组织特异性。Abstract: Objective: To explore the expression series of ID4 gene in normal and malignant tissues or cells as a primary work for studying the regulation mechanism of ID4 gene.Methods: Bioinformatics and free open data-set such as GeneBank, SAGE, GeneCard, GEO, InterPro, ProtoNet, UniProt and BLOCKS were used to analyze the expression series of ID4 mRNA and ID4 protein. Results: ID4 gene had higher expression in a lot of malignant neuro-tissues and cells than in normal brain tissues（209/3, 148/3, 123/3, 72/3, 64/3, 45/3）; higher in normal thyroid cells than in follicular thyroid carcinoma cells（172/35）, in metastasis and in situ breast carcinoma higher than normal breast （34/10, 36/10）, in normal lung tissues than lung adenocarcinoma（11/4）. Further more, ID4 gene had expression in normal bone marrow, lymphocyte and embryonic stem cell normal cell. Conclusion: Bioinformatics can be used freely to analyze a gene’s expression series. ID4 gene has different expression in different tissues.
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