Abstract: Objective: To develop a DNA vector-based RNA interference technology that inhibite the activity of the telomerase of the carcinoma cell in order to suppress the preliforation of the cells. Methods: A plasmid including U6 promoter and siRNA of hTERT was designed and constructed.The plasmid was transfected into ALVA-41 cell line.The telomerase activity was tested by telomerase repeat amplification protocol ELISA（TRAP-ELISA）. Cell calculating assay was used to assay the cell proliferation（activity）,and hTERT expression was assessed by Western blot. Results: The U6 expression plasmid that was constructed for hTERT showed obvious interfering effect.hTERT-siRNA could down regulate the expression of hTERT protein,inhibit telomerase activity and proliferation of ALVA-41 cells. Conclusion: siRNA of hTERT can inhibit the expression of human telomerase and proliferation of ALVA-41 cells.