Abstract: Objective: To explore the basic biologically characteristics and the intracellular distribution of LRP16 protein cloned by us. Methods: The primarily structural sequence of LRP16 protein was thoroughly analysed using public databases provided by Internet and biological softwares. On the basis of bioinformatical hint, LRP16 was recombined in pEGFP-N1 vector, inserting in the N-termini of GFP（green fluorescence protein）,and then transferred into HeLa cell line. Fluorescent signal was detected using confocal. Results: LRP16 protein has relatively higher similarity with some non-structural proteins coded by RNA virus, which are nucleic acid binding proteins. In addition,LRP16 has similar motif to the function-unknown Hismacro,DUF27 and A1pp.The green fluorescence was inspired from cellular nucleolus. Conclusion: LRP16 protein is a nuclear factor, which originated prior to the appearance of eukaryotes, and its evolution velocity is relatively slow and its motif has retained the basic function of ancestral gene. The motif feature provide a clue that LRP16 protein maybe play an important function in cellular multiple behavior.