Abstract: Objective: To study the neutralization effect of anti-human TNF-α monoclonal antibody on the TNF-α in sera of the psoriatic patients. Methods: We used sandwich ELISA to detect the concentrations of TNF-α in sera of healthy volunteers and patients before and after neutralization with antibody. L929 cells were Used to detect the bioactivities of TNF-α in sera of healthy volunteers and patients before and after neutralization with antibody. Results: The concentrations of TNF-α in sera of healthy volunteers and patients were（3.146±6.184） pg/ml and（62.723±38.379） pg/ml respectively;their bioactivities were（6.817±5.086） % and（57.498±9.125）% respectively. After neutralization with antibody, the concentrations of TNF-α of patients were（0.503±1.178）pg/ml, and the bioactivities of TNF-α of patients were（25.378±14.863）%. Conclusions: We can find that the concentrations and bioactivities of TNF-α in sera of patients were elevated, and anti-human TNF-α monoclonal antibody can well neutralize the concentrations and bioactivities of TNF-α in psoriatic patients’ sera.